An Enhanced Immune Response in Mice Lacking the Transcription Factor NFAT1

Abstract

Transcription factors of the NFAT family are thought to play a major role in regulating the expression of cytokine genes and other inducible genes during the immune response. The role of NFAT1 was investigated by targeted disruption of the NFAT1 gene. Unexpectedly, cells from NFAT1-'- mice showed increased primary responses to Leishmania major and mounted increased secondary responses to ovalbumin in vitro. In an in vivo model of allergic inflammation, the accumulation of eosinophils and levels of serum immunoglob ulin E were increased in NFAT1 '- mice. These results suggest that NFAT1 exerts a negative regulatory influence on the immune response.