Biochemical changes and physiological quality of corn seeds subjected to different chemical treatments and storage times

dc.TypeArticlept_BR
dc.contributor.authorOliveira, Thiago Lucas de
dc.contributor.authorPinho, Renzo Garcia Von
dc.contributor.authorSantos, Heloisa Oliveira dos
dc.contributor.authorSilva, Karen Marcelle de Jesus
dc.contributor.authorPereira, Elise de Matos
dc.contributor.authorSouza, João Lucas Dias
dc.date.accessioned2022-03-29T13:33:59Z
dc.date.available2022-03-29T13:33:59Z
dc.date.issued2020
dc.descriptionv.42, e202042038, 2020
dc.description.abstractO tratamento químico de sementes de milho é prática amplamente adotada para proteção das lavouras. No entanto, algumas moléculas interferem na qualidade das sementes, sobretudo quando armazenadas, acelerando sua deterioração. O objetivo deste trabalho foi investigar a influência de inseticidas sobre a expressão enzimática e qualidade de sementes de milho submetidas ao armazenamento. Sementes dos híbridos 2B647PW e SHS4070 foram submetidas a quatro diferentes tratamentos químicos (tratamento padrão, padrão + clotianidina, padrão + tiametoxam e padrão + fipronil) e armazenadas por diferentes períodos (0, 3, 6 e 9 meses). Os testes de qualidade incluíram a primeira contagem de germinação, germinação, teste frio sem solo e teste de sanidade. A expressão das enzimas α-amilase, esterase, superóxido dismutase, catalase e álcool desidrogenase foram determinadas pela técnica de eletroforese em gel. Concluiu-se que os inseticidas clotianidina, tiametoxam e fipronil reduzem a qualidade fisiológica das sementes do híbrido 2B647PW aos nove meses de armazenamento. O tratamento das sementes com os inseticidas clotianidina, tiametoxam e fipronil reduz a expressão das enzimas α-amilase, superóxido dismutase e catalase aos nove meses de armazenamento. As enzimas α-amilase, superóxido dismutase, catalase e álcool desidrogenase são bons marcadores da qualidade de sementes híbridas de milho.
dc.description.abstractenThe chemical treatment of corn seeds is widely adopted for protecting crops at the early stages. However, some molecules interfere in the quality of seeds, especially when stored, by accelerating their deterioration. The objective of this work was to investigate the influence of insecticides on the enzymatic expression and quality of corn seeds subjected to storage. Seeds of the hybrids 2B647PW and SHS4070 were subjected to four different chemical treatments (standard treatment, standard + clothianidin, standard + thiamethoxam, and standard + fipronil) and stored for different periods (0, 3, 6, and 9 months). The quality evaluation included the germination test, first germination count, cold test, and health test. The expression of the enzymes α-amylase, esterase, superoxide dismutase, catalase, and alcohol dehydrogenase were determined by the gel electrophoresis technique. The insecticides clothianidin, thiamethoxam, and fipronil reduce the physiological quality of the seeds of hybrid 2B647PW after nine months of storage. The treatment of seeds with the insecticides clothianidin, thiamethoxam, and fipronil reduces the expression of α-amylase, superoxide dismutase, and catalase enzymes after nine months of storage. The enzymes α-amylase, superoxide dismutase, catalase, and alcohol dehydrogenase are good quality markers for hybrid corn seeds.
dc.identifier.issn2317-1545
dc.identifier.urihttp://sr-vmlxaph03:8080/jspui/handle/123456789/6083
dc.publisherJournal of Seed Sciencept_BR
dc.subjectZea mayspt_BR
dc.subjectSementespt_BR
dc.subjectSeedspt_BR
dc.subjectTerapêuticapt_BR
dc.subjectTherapeuticspt_BR
dc.subjectEnzimaspt_BR
dc.subjectEnzymespt_BR
dc.subjectContaminação de Alimentospt_BR
dc.subjectFood Contaminationpt_BR
dc.titleBiochemical changes and physiological quality of corn seeds subjected to different chemical treatments and storage timespt_BR
dc.title.alternativeAlterações bioquímicas e qualidade fisiológica de sementes de milho submetidas a diferentes tratamentos químicos e tempos de armazenamentopt_BR

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