Improving the Phototoxicity of the Zinc Phthalocyanine by Encapsulation in Nanoparticles: Preparation, Characterization and Phototherapy Studies

Abstract

Nanoparticles are widely utilized to overcome drugs insolubility problems and sustain re lease improving the bioavailability. Zinc phthalocyanine, a hydrophobic photosensitizer with solubility problems, was loaded in PLA nanoparticles. Photosensitizer loaded in polymeric nanoparticles was pro duced with the following characteristics: size in the 200-300 nm range, negative zeta potential (-15 to -19 mV), low polydispersity index (< 0.1), satisfactory encapsulation efficiency (70-80%), low residual PVA, smooth surface and spherical shape. The photosensitizer release from nanoparticles was sustained and the kinetic followed Higuchi’s model. ZnPc loaded in polymeric nanoparticles exhibited higher phototoxicity than free photosensitizer. Phototoxicity of the ZnPc loaded in Resomer® R203 nanoparticles was im proved for increasing photosensitizer concentration (1 to 4 μg/ml), light dose (10 to 30 J/cm2) or incubation time (2 to 4 h). The phototoxicity of the zinc phthalocyanine was improved by encapsulation in nanoparti cles and this nanocarrier is a promising delivery system for photodynamic therapy use.