Coleção voltada para a inserção de documentos relacionados com pesquisa experimental e translacional https://ninho.inca.gov.br/jspui/handle/123456789/2181 2026-02-03T19:43:18Z 2026-02-03T19:43:18Z Pereira, Thais Hancio Mazzoccoli, Luciano Guimarães, Gustavo Henrique Cardoso Robaina, Marcela Cristina da Silva Mendonça, Bruna dos Santos Moraes, Gabriela Nestal de Monte-Mór, Bárbara da Costa Reis Gutiyama, Luciana Mayumi Carvalho, Luíze Otero de Daher Netto, Chaquip Costa, Paulo Roberto Ribeiro Faria, Fernanda Costas Casal de https://ninho.inca.gov.br/jspui/handle/123456789/13764 2024-04-11T15:08:25Z 2021-01-01T00:00:00Z

Title: The pterocarpanquinone LQB‑118 compound induces apoptosis of cytarabine‑resistant acute myeloid leukemia cells Authors: Pereira, Thais Hancio; Mazzoccoli, Luciano; Guimarães, Gustavo Henrique Cardoso; Robaina, Marcela Cristina da Silva; Mendonça, Bruna dos Santos; Moraes, Gabriela Nestal de; Monte-Mór, Bárbara da Costa Reis; Gutiyama, Luciana Mayumi; Carvalho, Luíze Otero de; Daher Netto, Chaquip; Costa, Paulo Roberto Ribeiro; Faria, Fernanda Costas Casal de Abstract: Acute myeloid leukemia (AML) is a complex hematological disorder characterized by blockage of differentiation and high proliferation rates of myeloid progenitors. Anthracycline and cytarabine‑based therapy has remained the standard treatment for AML over the last four decades. Although this treatment strategy has increased survival rates, patients often develop resistance to these drugs. Despite efforts to understand the mechanisms underlying cytarabine resistance, there have been few advances in the field. The present study developed an in vitro AML cell line model resistant to cytarabine (HL‑60R), and identified chromosomal aberrations by karyotype evaluation and potential molecular mechanisms underlying chemoresistance. Cytarabine decreased cell viability, as determined by MTT assay, and induced cell death and cell cycle arrest in the parental HL‑60 cell line, as revealed by Annexin V/propidium iodide (PI) staining and PI DNA incorporation, respectively, whereas no change was observed in the HL‑60R cell line. In addition, the HL‑60R cell line exhibited a higher tumorigenic capacity in vivo compared with the parental cell line. Notably, no reduction in tumor volume was detected in mice treated with cytarabine and inoculated with HL‑60R cells. In addition, western blotting revealed that the protein expression levels of Bcl‑2, X‑linked inhibitor of apoptosis protein (XIAP) and c‑Myc were upregulated in HL‑60R cells compared with those in HL‑60 cells, along with predominant nuclear localization of the p50 and p65 subunits of NF‑κB in HL‑60R cells. Furthermore, the antitumor effect of LQB‑118 pterocarpanquinone was investigated; this compound induced apoptosis, a reduction in cell viability and a decrease in XIAP expression in cytarabine‑resistant cells. Taken together, these data indicated that acquired cytarabine resistance in AML was a multifactorial process, involving chromosomal aberrations, and differential expression of apoptosis and cell proliferation signaling pathways. Furthermore, LQB‑118 could be a potential alternative therapeutic approach to treat cytarabine‑resistant leukemia cells. Description: p. 1-14.: il. p&b. e color.

2021-01-01T00:00:00Z Aguiar, Flávia Rhana, Paula Bloise, Enrrico Nunes, Cristiana Buzelin Rodrigues, Andreia Laura Ferreira, Enio https://ninho.inca.gov.br/jspui/handle/123456789/13760 2023-05-10T15:54:49Z 2023-01-01T00:00:00Z

Title: T-type Ca2+ channels and their relationship with pre-neoplastic and neoplastic lesions in the human breast Authors: Aguiar, Flávia; Rhana, Paula; Bloise, Enrrico; Nunes, Cristiana Buzelin; Rodrigues, Andreia Laura; Ferreira, Enio Abstract: The expression of T-type voltage-dependent Ca2+ channels (Cav3) has been previously observed in breast cancer, but their expression and subcellular localization were not evaluated in pre-neoplastic lesions. Therefore, this work aimed to evaluate protein expression and subcellular localization of T-type channel isoforms in human breast tissue samples. Protein expressions of CaV3.1, CaV3.2, and CaV3.3 were evaluated by immunohistochemistry in breast without alteration, in proliferative non-neoplastic lesions, and in neoplastic ductal epithelial lesions of the human breast. CaV3.1, CaV3.2, and CaV3.3 nuclear expressions were decreased in advanced stages of neoplastic transformation, whereas CaV3.1 and CaV3.2 cytoplasmic expression increased. Also, the decrease in nuclear expression was correlated with an increase in cytoplasmic expression for CaV3.1 isoform. The change in CaV3 protein expression and subcellular localization are consistent with the neoplastic transformation stages of mammary epithelial cells, evident in early neoplastic lesions, such as ductal carcinomas in situ. These results suggest a possible involvement of CaV3 in the carcinogenic processes and could be considered as a potential pharmacological target in new therapies for breast cancer treatment. Description: v. 56, 2023.

2023-01-01T00:00:00Z Bastos, Ana Clara Santos da Fonseca Gomes, Amanda Vitória Pampolha Silva, Gabriela Ribeiro Emerenciano, Mariana Ferreira, Luciana Bueno Gimba, Etel Rodrigues Pereira https://ninho.inca.gov.br/jspui/handle/123456789/13731 2023-05-08T20:03:39Z 2023-01-01T00:00:00Z

Title: The intracellular and secreted sides of osteopontin and their putative physiopathological roles Authors: Bastos, Ana Clara Santos da Fonseca; Gomes, Amanda Vitória Pampolha; Silva, Gabriela Ribeiro; Emerenciano, Mariana; Ferreira, Luciana Bueno; Gimba, Etel Rodrigues Pereira Abstract: Classically, osteopontin (OPN) has been described as a secreted glycophosprotein. Indeed, most data concerning its physiological and pathological roles are mainly related to the secreted OPN (sOPN). However, there are several instances in which intracellular OPN (iOPN) has been described, presenting some specific roles in distinct experimental models, such as in the immune system, cancer cells, and neurological disorders. We herein aimed to highlight and discuss some of these secreted and intracellular roles of OPN and their putative clinical and biological impacts. Moreover, by consolidating data from the OPN protein database, we also analyzed the occurrence of signal peptide (SP) sequences and putative subcellular localization, especially concerning currently known OPN splicing variants (OPN-SV). Comprehending the roles of OPN in its distinct cellular and tissue environments may provide data regarding the additional applications of this protein as biomarkers and targets for therapeutic purposes, besides further describing its pleiotropic roles. Description: v. 24, n. 3, p. 2942, fev. 2023

2023-01-01T00:00:00Z Castro, Claudia Garcia Serpa Osorio de Santos, Ranailla Lima Bandeira dos Silva, Mario Jorge Sobreira da Pepe, Vera Lúcia Edais https://ninho.inca.gov.br/jspui/handle/123456789/13728 2023-06-28T19:09:12Z 2023-01-01T00:00:00Z

Title: First use of antineoplastic agents in women with breast cancer in the state of Rio de Janeiro, Brazil Authors: Castro, Claudia Garcia Serpa Osorio de; Santos, Ranailla Lima Bandeira dos; Silva, Mario Jorge Sobreira da; Pepe, Vera Lúcia Edais Abstract: Context: Breast cancer is the most common cancer, except for non-melanoma skin cancer, among women in Brazil and worldwide. Breast cancer treatment involves surgery, radiotherapy and chemotherapy, which is used in 70% of patients. This study analyzes the utilization of antineoplastic agents among women undergoing their first round of chemotherapy in Brazil's public health system (SUS) in the state of Rio de Janeiro. Methods: Data from the SUS Outpatient Information System's authorizations for high-complexity outpatient procedures (APACs) billed between January 2013 and December 2019 were extracted, and three datasets were created: all type 1 and type 2 APACs (including all chemotherapy procedures performed); all type 1 APACs; and first type 1 APACs (containing data only for the first round of breast cancer chemotherapy). Names of antineoplastic agents were standardized to enable the subsequent classification of therapy regimens, mitigating limitations related to data quality. Absolute and relative frequencies were used to describe sociodemographic, clinical and treatment characteristics, therapy regimen and supportive drugs. Results: We analyzed 23,232 records of women undergoing their first round of chemotherapy. There was a progressive increase in the number of procedures over time. Women were predominantly white, lived in the capital and close to the treatment center. Most had stage 3 cancer at diagnosis (50.51%) and a significant proportion had regional lymph node invasion (37.9%). The most commonly used chemotherapy regimens were TAC (docetaxel, doxorubicine, cyclophosphamide) (21.05%) and and cyclophosphamide (17.71%), followed by tamoxifen (15.65%) and anastrozole (12.94%). Supportive drugs were prescribed to 386 women and zoledronic acid was predominant (59.58%). Conclusion: The findings point to important bottlenecks and possible inequities in access to treatment and medicine utilization for breast cancer patients in Brazil. Efforts to improve breast cancer treatment and prevention should not only focus on interventions at the individual level but address the disease as a public health problem. The study focused on women undergoing their first round of treatment, providing valuable insight into patient and treatment characteristics to inform policy decisions. Description: v. 14, fev. 2023.

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