Coleção voltada para a inserção de artigos de periódicos desenvolvidos pela área tecido ósseo e conectivo, seus servidores e/ou colaboradores. https://ninho.inca.gov.br/jspui/handle/123456789/5175 2026-04-19T07:50:21Z 2026-04-19T07:50:21Z Pereira, Thais Hancio Mazzoccoli, Luciano Guimarães, Gustavo Henrique Cardoso Robaina, Marcela Cristina da Silva Mendonça, Bruna dos Santos Moraes, Gabriela Nestal de Monte-Mór, Bárbara da Costa Reis Gutiyama, Luciana Mayumi Carvalho, Luíze Otero de Daher Netto, Chaquip Costa, Paulo Roberto Ribeiro Faria, Fernanda Costas Casal de https://ninho.inca.gov.br/jspui/handle/123456789/13764 2024-04-11T15:08:25Z 2021-01-01T00:00:00Z

Title: The pterocarpanquinone LQB‑118 compound induces apoptosis of cytarabine‑resistant acute myeloid leukemia cells Authors: Pereira, Thais Hancio; Mazzoccoli, Luciano; Guimarães, Gustavo Henrique Cardoso; Robaina, Marcela Cristina da Silva; Mendonça, Bruna dos Santos; Moraes, Gabriela Nestal de; Monte-Mór, Bárbara da Costa Reis; Gutiyama, Luciana Mayumi; Carvalho, Luíze Otero de; Daher Netto, Chaquip; Costa, Paulo Roberto Ribeiro; Faria, Fernanda Costas Casal de Abstract: Acute myeloid leukemia (AML) is a complex hematological disorder characterized by blockage of differentiation and high proliferation rates of myeloid progenitors. Anthracycline and cytarabine‑based therapy has remained the standard treatment for AML over the last four decades. Although this treatment strategy has increased survival rates, patients often develop resistance to these drugs. Despite efforts to understand the mechanisms underlying cytarabine resistance, there have been few advances in the field. The present study developed an in vitro AML cell line model resistant to cytarabine (HL‑60R), and identified chromosomal aberrations by karyotype evaluation and potential molecular mechanisms underlying chemoresistance. Cytarabine decreased cell viability, as determined by MTT assay, and induced cell death and cell cycle arrest in the parental HL‑60 cell line, as revealed by Annexin V/propidium iodide (PI) staining and PI DNA incorporation, respectively, whereas no change was observed in the HL‑60R cell line. In addition, the HL‑60R cell line exhibited a higher tumorigenic capacity in vivo compared with the parental cell line. Notably, no reduction in tumor volume was detected in mice treated with cytarabine and inoculated with HL‑60R cells. In addition, western blotting revealed that the protein expression levels of Bcl‑2, X‑linked inhibitor of apoptosis protein (XIAP) and c‑Myc were upregulated in HL‑60R cells compared with those in HL‑60 cells, along with predominant nuclear localization of the p50 and p65 subunits of NF‑κB in HL‑60R cells. Furthermore, the antitumor effect of LQB‑118 pterocarpanquinone was investigated; this compound induced apoptosis, a reduction in cell viability and a decrease in XIAP expression in cytarabine‑resistant cells. Taken together, these data indicated that acquired cytarabine resistance in AML was a multifactorial process, involving chromosomal aberrations, and differential expression of apoptosis and cell proliferation signaling pathways. Furthermore, LQB‑118 could be a potential alternative therapeutic approach to treat cytarabine‑resistant leukemia cells. Description: p. 1-14.: il. p&b. e color.

2021-01-01T00:00:00Z Garnica, Marcia Valetim, Marcia Rejane Furtado, Paulo Moreira, Maria Cláudia Rodrigues Bigni, Ricardo de Sá Vinhas, Simone Dias, Paulo Cesar Santos Fellows, Ilza Boeira Martins, Wolney de Andrade https://ninho.inca.gov.br/jspui/handle/123456789/13703 2024-04-11T15:31:16Z 2020-01-01T00:00:00Z

Title: COVID-19 in hematology: data from a hematologic and transplant unit Authors: Garnica, Marcia; Valetim, Marcia Rejane; Furtado, Paulo; Moreira, Maria Cláudia Rodrigues; Bigni, Ricardo de Sá; Vinhas, Simone; Dias, Paulo Cesar Santos; Fellows, Ilza Boeira; Martins, Wolney de Andrade Abstract: During the COVID-19 pandemic, special attention has been addressed in cancer care to mitigate the impact on the patient’s prognosis. We addressed our preparation to face COVID-19 pandemic in a Hematological and Stem Cell Transplant Unit in Brazil during the first two months of COVID-19 pandemic and described COVID-19 cases in patients and health care workers (HCW). Modifications in daily routines included a separation of area and professionals, SARS-CoV-2 screening protocols, and others. A total of 47 patients and 54 HCW were tested for COVID-19, by PCR-SARS-CoV-2. We report 11 cases of COVID-19 in hematological patients (including 2 post stem cell transplant) and 28 cases in HCW. Hematological cases were most severe or moderate and presented with several poor risk factors. Among HCW, COVID-19 were mostly mild, and all recovered without hospitalization. A cluster was observed among HCW. Despite a decrease in the number of procedures, the Transplant Program performed 8 autologous and 4 allogeneic SCT during the period, and 49 onco-hematological patients were admitted to continuing their treatments. Although we observed a high frequency of COVID-19 among patients and HCW, showing that SARS-CoV-2 is disseminated in Brazil, hematological patients were safely treated during pandemic times. Description: p. 293-299.: il. p&b.

2020-01-01T00:00:00Z Fagundes, Evandro Maranhão Martins, Náira Neves Neto Caldas, Lorena Menezes Aragão, Jordana Santos Ramires Glória, Ana Beatriz Firmato Leite, Leonardo Gomes Teixeira Bastos, Paula Cardoso Rolo, Carolina de Melo Fernandes Chalup, Mariana Motta Baeta Salvador Vasconcelos, Rosa Arcuri Fagundes, Olivia Guimarães https://ninho.inca.gov.br/jspui/handle/123456789/13612 2024-04-11T15:06:37Z 2021-01-01T00:00:00Z

Title: Mortality by COVID-19 in adults with acute myeloid leukemia: a survey with hematologists in Brazil Authors: Fagundes, Evandro Maranhão; Martins, Náira Neves Neto; Caldas, Lorena Menezes; Aragão, Jordana Santos Ramires; Glória, Ana Beatriz Firmato; Leite, Leonardo Gomes Teixeira; Bastos, Paula Cardoso; Rolo, Carolina de Melo Fernandes; Chalup, Mariana Motta Baeta Salvador; Vasconcelos, Rosa Arcuri; Fagundes, Olivia Guimarães Abstract: COVID-19 caused by the novel coronavirus SARS-COV-2 and frst detected in Wuhan, China in 2019, has become one of the worst pandemics in history [1, 2]. Although approximately 50% of the infected individuals are asymptomatic, some may develop severe cytokine release syndrome with intense immune response and endothelial damage [3]. Obesity, hypertension, diabetes, COPD, advanced age, and malignancy have been listed as risk factors for severe disease, hospitalization, admission in intensive care unit, and death [4, 5]. Patients with hematological malignancies also have a higher risk of severe events and among all of them, acute myeloid leukemia (AML) seems to have the highest risk for death by COVID-19 [6]. Because avoiding medical services visits or prolonged stays in hospital is not an option for AML patients, these individuals have a higher chance of getting in contact and being infected by SARS-COV-2. Once infected, these patients may be at high risk for severe complications and death as they have immunosuppression, neutropenia, and epithelial mucosal barrier damage [7]. AML afects individuals of all ages whom may be in diferent hematological scenarios such as in remission or with active disease that may be treated with intensive chemotherapy or low intensity treatment. The impact of COVID-19 in those diferent AML scenarios is unknown. Our aim is to report a survey conducted with Brazilian hematologists who attended AML patients with COVID-19, to evaluate the mortality rate seen by those doctors among their patients and any potential risk factor for death. Description: p. 923-925.

2021-01-01T00:00:00Z Oliveira, Felipe Lameirão de Leira, Flávio do Nascimento Zamprogno, Pedro Henrique Aguiar Souto, Antonio Aversa Dutra do Silva, Raquel Guimarães Domingos da https://ninho.inca.gov.br/jspui/handle/123456789/13472 2024-04-11T15:17:05Z 2021-01-01T00:00:00Z

Title: Extradural arachnoid cyst: case report and literature review Authors: Oliveira, Felipe Lameirão de; Leira, Flávio do Nascimento; Zamprogno, Pedro Henrique Aguiar; Souto, Antonio Aversa Dutra do; Silva, Raquel Guimarães Domingos da Abstract: Extradural arachnoid cysts (EAC) are a rare entity that may cause spinal cord or radicular nerve compression, usually in young adults. The most common locations are the posterior or posterolateral region of the thoracic spinal canal or the thoracolumbar junction. The most accepted theory is that they are formed by the protrusion of the arachnoid through a defect in the dura, they progressively increase in size through physiological Valsalva maneuvers performed dailyand then they can become symptomatic by direct neural compression. Surgery is the treatment of choice for these cases, with complete removal of the cyst and closure of the dural defect. Extradural arachnoid cysts have generally a good prognosis. This article aims to present a case of an extradural arachnoid cyst in a 13 year old patient with spinal cord compression syndrome (paraparesis with hyperreflexia, clonus and alteration of deep sensitivity in the lower limbs), without previous history of trauma. Dorsal spine magnetic resonance imaging revealed an extradural cystic lesion with significant spinal cord compression similar to the CSF in T2 weighted images. The lesion extended from T6 to T9. Description: p. 1-4.: il. color.

2021-01-01T00:00:00Z