Please use this identifier to cite or link to this item: https://ninho.inca.gov.br/jspui/handle/123456789/12490
Title: Modulation of expression and activity of cytochrome P450s and alteration of praziquantel kinetics during murine schistosomiasis
Authors: Rojas, Mara Andréia Gotardo
Hyssa, Juliana
Carvalho, Renato Sampaio
Carvalho, Rosângela Ribeiro de
Motta, Luciana Soares Gueiros da
Siqueira, Carolina
Mello, Marcia Sarpa de Campos
Oliveira, Ana Cecilia Amado Xavier de
Paumgartten, Francisco José Roma
Keywords: Schistosoma mansoni
Redes e Vias Metabólicas
Metabolic Networks and Pathways
Farmacocinética
Pharmacokinetics
Sistema Enzimático do Citocromo P-450
Cytochrome P-450 Enzyme System
Issue Date: Mar-2011
Publisher: Memórias do Instituto Oswaldo Cruz, 2011 Mar;106(2):212-9
Abstract: In this study, we investigated the expression and activity of liver cytochrome P450s (CYPs) and praziquantel (PZQ) kinetics in mice infected with Schistosoma mansoni. Swiss Webster (SW) mice of both genders were infected (100 cercariae) on postnatal day 10 and killed on post-infection days (PIDs) 30 or 55. Non-infected mice of the same age and sex served as controls. Regardless of mouse sex, infection depressed the activities of CYP1A [ethoxy/ methoxy-resorufin-O-dealkylases (EROD/MROD)], 2B9/10 [pentoxy/benzyloxy-resorufin-O-dealkylases (PROD, BROD)], 2E1 [p-nitrophenol-hydroxylase (PNPH)] and 3A11 [erythromycin N-demethylase (END)] on PID 55 but not on PID 30. On PID 55, infection decreased liver CYP mRNA levels (real-time reverse transcription-polymerase chain reaction). On PID 30, whereas mRNA levels remained unaltered in males, they were depressed in females. Plasma PZQ (200 and 400 mg/kg body weight intraperitoneally) levels were measured (high-performance liquid chromatography) at different post-treatment intervals. In males and females, infection delayed the PZQ clearance on PID 55, but not on PID 30. Therefore, it can be concluded that schistosomiasis down-modulated CYP expression and activity and delayed PZQ clearance on PID 55, when a great number of parasite eggs were lodged in the liver. On PID 30, when egg-laying was initiated by the worms, no change of CYP expression and activity was found, except for a depression of CYP1A2 and 3A11 mRNAs in female mice.
URI: https://ninho.inca.gov.br/jspui/handle/123456789/12490
ISSN: 1678-8060
Appears in Collections:Artigos de Periódicos da área da Vigilância do Câncer Relacionado ao Trabalho e ao Ambiente
Marcia Sarpa de Campos Mello



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