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https://ninho.inca.gov.br/jspui/handle/123456789/13732
Title: | Naso-oropharyngeal microbiome from breast cancer patients diagnosed with COVID-19 |
Authors: | Alves, Maria Carolina Viana Alves Curty, Gislaine Furtado, Carolina Singh, Bhavya Bendall, Matthew Lewis Viola, Joao Paulo de Biaso Melo, Andreia Cristina de Soares, Marcelo Alves Moreira, Miguel Angelo Martins Tumor Genetics and Virology Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil. Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY, United States. Program of Immunology and Tumor Biology, Instituto Nacional de Câncer, Rio de Janeiro, Brazil. Division of Clinical Research and Technological Development, Instituto Nacional de Câncer, Rio de Janeiro, Brazil. |
Keywords: | COVID-19 Neoplasias da Mama Breast Neoplasms Neoplasias de la Mama |
Issue Date: | 2023 |
Publisher: | Frontiers In Microbiology |
Citation: | VIANA, Maria Carolina; CURTY, Gislaine; FURTADO, Carolina; SINGH, Bhavya; BENDALL, Matthew L.; VIOLA, João P. B.; MELO, Andreia Cristina de; SOARES, Marcelo A.; MOREIRA, Miguel A. M.. Naso-oropharyngeal microbiome from breast cancer patients diagnosed with COVID-19. Frontiers In Microbiology, [S.L.], v. 13, jan. 2023. DOI: http://dx.doi.org/10.3389/fmicb.2022.1074382. |
Abstract: | Due to immunosuppressive cancer therapies, cancer patients diagnosed with COVID-19 have a higher chance of developing severe symptoms and present a higher mortality rate in comparison to the general population. Here we show a comparative analysis of the microbiome from naso-oropharyngeal samples of breast cancer patients with respect to SARS-CoV-2 status and identified bacteria associated with symptom severity. Total DNA of naso-oropharyngeal swabs from 74 women with or without breast cancer, positive or negative for SARS-CoV-2 were PCR-amplified for 16S-rDNA V3 and V4 regions and submitted to massive parallel sequencing. Sequencing data were analyzed with QIIME2 and taxonomic identification was performed using the q2-feature-classifier QIIME2 plugin, the Greengenes Database, and amplicon sequence variants (ASV) analysis. A total of 486 different bacteria were identified. No difference was found in taxa diversity between sample groups. Cluster analysis did not group the samples concerning SARS-CoV-2 status, breast cancer diagnosis, or symptom severity. Three taxa (Pseudomonas, Moraxella, and Klebsiella,) showed to be overrepresented in women with breast cancer and positive for SARS-CoV-2 when compared to the other women groups, and five bacterial groups were associated with COVID-19 severity among breast cancer patients: Staphylococcus, Staphylococcus epidermidis, Scardovia, Parasegitibacter luogiensis, and Thermomonas. The presence of Staphylococcus in COVID-19 breast cancer patients may possibly be a consequence of nosocomial infection. |
Description: | v. 13, jan. 2023. |
URI: | https://ninho.inca.gov.br/jspui/handle/123456789/13732 |
ISSN: | 1664-3224 |
Appears in Collections: | Artigo de Periódicos da Pesquisa Clínica |
Files in This Item:
File | Description | Size | Format | |
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Naso-oropharyngeal microbiome from breast cancer patients diagnosed with COVID-19 - 2023.pdf | 1.09 MB | Adobe PDF | View/Open |
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