Please use this identifier to cite or link to this item: https://ninho.inca.gov.br/jspui/handle/123456789/13881
Title: Bone marrow stroma inhibits proliferation and apoptosis in leukemic cells through gap junction-mediated cell communication
Authors: Braga, Flavio Henrique Paraguassu
Borojevic, Radovan
Bouzas, Luis Fernando da Silva
Barcinski, Marcello Andre
Bonomo, Adriana Cesar
Keywords: Junções Comunicantes
Gap Junctions
Conexinas
Connexins
Leucemia
Leukemia
Células-Tronco
Stem Cells
Proliferação de Células
Cell Proliferation
Apoptose
Apoptosis
Issue Date: Sep-2003
Abstract: Normal and leukemic blood cell progenitors depend upon the bone marrow (BM) stroma with which they communicate through soluble and membrane-anchored mediators, adhe sive interactions and gap junctions (GJ). Regarding hemato poiesis, it is believed that it can be influenced by connexin expression, but the exact role of GJ in cell death and proliferation is not clear. Using flow cytometry, we monitored the division rate of leukemic cell lines, communicating and not communicating with stromal cell line through GJ. We found that GJ-coupled cells (i) did not proliferate; (ii) were kept in G0; and (iii) were protected from drug-induced apoptosis when compared to either total or uncoupled cell population. We conclude that GJ coupling between stroma and leukemic lymphoblasts prevents proliferation, keeping cells in a quiescent state, thus increasing their resistance to antimitotic drugs. Since GJ are particularly abundant in the sub-endosteal environment, which harbors blood stem cells, we also asked which cells within the normal human BM communicate with the stroma. Using a primary BM stroma cell culture, our results show that 80% of CD34 þ progenitors communicate through GJ. We propose that blood cell progenitors might be retained in the low-cycling state by GJ-mediated communication with the hematopoietic stroma.
URI: https://ninho.inca.gov.br/jspui/handle/123456789/13881
ISSN: 1476-5403
Appears in Collections:Hospital do Câncer I (HCI)



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