Please use this identifier to cite or link to this item: https://ninho.inca.gov.br/jspui/handle/123456789/13733
Title: Procoagulant properties of human MV3 melanoma cells
Authors: Silva, Renato Fernandes da
Fidalgo, Thereza Christina Barja
Monteiro, Robson de Queiroz
Adriano, Diego Geaquinto Leão
Keywords: MV3 melanoma
Blood Coagulation
Coagulação Sanguínea
Thromboplastin
Tromboplastina
Thrombin
Trombina
Issue Date: 2008
Publisher: Brazilian Journal of Medical and Biological Research
Citation: SILVA, Renato Fernandes da et al. Procoagulant properties of human MV3 melanoma cells. Brazilian Journal of Medical and Biological Research, v. 41, p. 99-105, 2008.
Abstract: A correlation between cancer and prothrombotic states has long been described. More recently, a number of studies have focused on the procoagulant mechanisms exhibited by tumor cells. In the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of MV3, a highly aggressive human melanoma cell line. It was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor Xa formation in the presence of cells and factor VIIa), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, S-2238, and the natural substrate, fibrinogen. This ability was highly potentiated by the addition of exogenous factor Va, which functions as a cofactor for the enzyme factor Xa. In contrast, prothrombin activation was not observed when cells were previously incubated with DEGR-factor Xa, an inactive derivative of the enzyme. Moreover, a monoclonal antibody against bovine factor Xa reduced the prothrombin-converting activity of tumor cells. In conclusion, the data strongly suggest that MV3 cells recruit factor Xa from the culture medium, triggering an uncommon procoagulant mechanism.
Description: p. 99-105.: il. p&b.
URI: https://ninho.inca.gov.br/jspui/handle/123456789/13733
ISSN: 0100-879X
Appears in Collections:Artigos de Periódicos da área de Enfermagem

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