Please use this identifier to cite or link to this item: https://ninho.inca.gov.br/jspui/handle/123456789/13933
Title: SUZ12 Is a Candidate Target of the Non-canonical WNT Pathway in the Progression of Chronic Myeloid Leukemia
Authors: Pizzatti, Luciana
Binato, Renata
Cofre, Jaime
Gomes, Bernadete Evangelho
Dobbin, Jane de Almeida
Haussmann, Maria Emilia
Ramos, Denize D' Azambuja
Bouzas, Luis Fernando da Silva
Keywords: Leucemia Mieloide
Leukemia Myeloid
Progressão da Doença
Disease Progression
Proteínas Nucleares
Nuclear Proteins
Estudos de Associação Genética
Genetic Association Studies
Proteínas Wnt
Wnt Proteins
Issue Date: Feb-2010
Abstract: Polycomb proteins form multiprotein complexes that repress target genes by chromatin remodeling. In this work, we report that the SUZ12 polycomb gene is over-expressed in bone marrow samples of patients at the blastic phase of chronic myeloid leukemia. We also found a direct interaction between polycomb group genes and the WNT signaling path way in chronic myeloid leukemia transformation. Electrophoretic mobility shift assay (EMSA), Chromatin immunoprecipita tion assay (ChIP), and mass spectrometry assays identified noncanonical WNT pathway members, such as WNT5A and WNT11, bound to the SUZ12 promoter. Immunohistochemistry and immunofluorescence with WNT5A and WNT11 anti bodies confirmed nuclear localization. Knockdown of WNTs 1, 5A, and 11 with RNAi approaches showed that WNT members are capable of activating SUZ12 transcription with varying promoter affinities. Finally, we suggest that SUZ12 is blocking cellular differentiation, as SUZ12 knockdown release differentiation programs in chronic myeloid blastic phase (CML-BP) transformed cell line.
URI: https://ninho.inca.gov.br/jspui/handle/123456789/13933
ISSN: 1098-2264
Appears in Collections:Hospital do Câncer I (HCI)

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