Please use this identifier to cite or link to this item: https://ninho.inca.gov.br/jspui/handle/123456789/14281
Title: Suramin counteracts the haemostatic disturbances produced by bothrops jararaca snake venom
Authors: Silva, Renato Fernandes da
Assafim, Mariane Inês D'Avila
Arruda, Emerson Zumpichiatti
Melo, Paulo de Assis
Zingali, Russolina Benedeta
Monteiro, Robson de Queiroz
Keywords: Suramin
Suramina
Crotalid Venoms
Venenos de Crotalídeos
Venous Thrombosis
Trombose Venosa
Antibothropic Serum
Soro Antibotrópico
Issue Date: 2007
Publisher: Toxicon
Citation: SILVA, Renato Fernandes da et al. Suramin counteracts the haemostatic disturbances produced by bothrops jararaca snake venom. Toxicon, v. 49, p. 931–938, 2007.
Abstract: Snakebite accidents produced by Bothrops jararaca typically results in haemostatic changes including pro- and anticoagulant disturbs as well as interference with platelets. Suramin is a hexasulfonated naphthylurea derivative that was recently characterized as a thrombin inhibitor (Monteiro et al., 2004. Suramin interaction with human alpha-thrombin: inhibitory effects and binding studies. Int. J. Biochem. Cell Biol. 36(10), 2077–2085). Here, we evaluated the ability of suramin to counteract some of the haemostatic disturbs produced by B. jararaca venom. In vitro assays showed that suramin inhibited venom-induced hydrolysis of a number of synthetic substrates: S-2238, S-2266, S-2302 and S-2288, being this ability more prominent towards the thrombin substrate S-2238 (IC50 ¼ 4.3 mM). It was also observed that suramin impaired the fibrinogen clotting induced by B. jararaca venom (IC50 ¼ 124 mM). Accordingly, increasing concentrations of suramin progressively delayed venom-induced plasma clotting, with complete inhibition attained at concentrations above 1.0 mM. In addition, the platelet-aggregating properties of B. jararaca venom were inhibited by suramin in a dose dependent fashion (IC50 ¼ 127 mM). Suramin showed no effect in the in vivo hemorrhagic effect of venom in mouse skin. The in vivo effect of suramin was further tested using a previously established venous thrombosis model in rats induced by intravenous administration of B. jararaca venom combined with stasis. Venom doses of 100 mg/kg produced 100% of thrombus incidence (10.671.7 mg). On the other hand, previous administration of suramin partially inhibited thrombus formation. Thus, 12.5 or 25 mg/kg of suramin decreased thrombus weight by 24% and 40%, respectively. Remarkably, co administration of 3 mL/kg of antibothropic serum (which has no effect on thrombus formation) and 12.5 mg/kg of suramin decreased thrombus weight by 75%, suggesting a synergic effect. Altogether, we demonstrate here that suramin inhibits in vitro and in vivo haemostatic changes caused by B. jararaca venom. At this point, this drug could be of potential interest for association with conventional antiserum therapy. r 2007 Elsevier Ltd. All rights reserved.
Description: p. 931–938.: il. p&b.
URI: https://ninho.inca.gov.br/jspui/handle/123456789/14281
ISSN: 1879-3150
Appears in Collections:Artigos de Periódicos da área de Enfermagem

Files in This Item:
File Description SizeFormat 
Suramin counteracts the haemostatic disturbances produced by bothrops jararaca snake venom.pdf172.42 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.