Please use this identifier to cite or link to this item: https://ninho.inca.gov.br/jspui/handle/123456789/6541
Title: Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus
Authors: Cunha, Paula Rêgo Bittencourt
Cardoso, Livia Silva
Oliveira, Giselle de Almeida
Silva, José Roberto da
Silveira, Alan Barbosa da
Kluck, George Eduardo Gabriel
Lima, Michele Souza
Gondim, Katia Calp
Silva, Carlos Peres
Masuda, Hatisaburo
Silva Neto, Mario Alberto Cardoso da
Atella, Geórgia Correa
Alencar, Marilvia Dansa de
Keywords: Lipid Metabolism
Metabolismo dos Lipídeos
Lipoproteins
Lipoproteínas
Lifting
Remoção
Lipids
Lipídeos
Phospholipids
Fosfolipídeos
Gastrula
Gástrula
Issue Date: 2013
Publisher: Mem Inst Oswaldo Cruz
Citation: CUNHA, Paula Rêgo Bittencourt et al. Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus. Mem Inst Oswaldo Cruz, Rio de Janeiro, v. 108, n. 4, p. 494-500, june 2013.
Abstract: In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs.
Description: p. 494-500.: il. p&b.
URI: http://sr-vmlxaph03:8080/jspui/handle/123456789/6541
ISSN: 1678-8060
Appears in Collections:Artigos de Periódicos da área de Farmácia



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